Membrane filtration (MF) is the most accurate method to determine the number of indicator bacteria in a water sample and is internationally recognized as a standard method for water quality testing. Membrane filtration can be done in a laboratory or by using a portable test kit as demonstrated in this video.
In general, a 100 mL water sample is filtered through filter paper using a small hand pump. After filtration, the filter paper is placed in a Petri dish with a nutrient solution and incubated at a specific temperature and time. After incubation, the bacteria colonies are counted to determine the number of colony forming units (CFU) per 100 mL.
Membrane filtration (MF) is the most accurate method to determine the number of indicator bacteria in a water sample and is internationally recognized as a standard method for water quality testing. Membrane filtration can be done in a laboratory or by using a portable test kit as demonstrated in this video.
In general, a 100 mL water sample is filtered through filter paper using a small hand pump. After filtration, the filter paper is placed in a Petri dish with a nutrient solution and incubated at a specific temperature and time. After incubation, the bacteria colonies are counted to determine the number of colony forming units (CFU) per 100 mL.
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